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8 What structure is formed by two sister chromatids being held together by a centromere. That holds together the two chromatids the daughter strands of a replicated chromosome. Chromatid What structure holds the two sister chromatids together as they prepare for cell division. . The link between the two sister chromatids is broken at the anaphase stage of the cell division when spindle fibers pull them apart towards opposite poles within the cell. Describe or explain sister chromatids by addressing the following questions. CHROMOSOMAL STRUCTURE The two chromatids of a duplicated chromosome are held together at a region of DNA called the centromere see figure below. No the centromere only serves to hold two sister chromatids together. How many centromere hold together two chromatids. Sister chromatids are held together by a protein called Cohesin at a number of places along the length of the two chromatids. ...